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dapi staining reagent  (Servicebio Inc)

 
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    Servicebio Inc dapi staining reagent
    Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence <t>after</t> <t>staining</t> with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by <t>DAPI</t> (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001
    Dapi Staining Reagent, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi staining reagent/product/Servicebio Inc
    Average 86 stars, based on 1 article reviews
    dapi staining reagent - by Bioz Stars, 2026-05
    86/100 stars

    Images

    1) Product Images from "PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia"

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia

    Journal: Inflammation

    doi: 10.1007/s10753-026-02476-9

    Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence after staining with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by DAPI (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001
    Figure Legend Snippet: Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence after staining with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by DAPI (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001

    Techniques Used: Flow Cytometry, Fluorescence, Immunofluorescence, Staining

    Hyperoxia induces the increase in glycolysis levels in the lungs of BPD mice. a : Detect the lactate levels in the BALF of N7, H7, N14 and H14 groups. b : The mRNA expression level of PKM2 quantified by RT-qPCR. c : The activity of HK in the lung tissue of N7, H7, N14 and H14 groups. d : The protein levels of PKM2 in the lung tissue of N7, H7, N14 and H14 groups were detected by WB. e : Representative images of immunofluorescence after staining with anti-CD86 antibody (green) and anti-PKM2 antibody (red) at 14 days. Nuclei were stained by DAPI (blue). f : The relative fluorescence intensity of PKM2 in the lungs of each group at 14 days. Data are shown as means ± SD ( n = 3). * P < 0.05, ** P <0.01, *** P<0.001
    Figure Legend Snippet: Hyperoxia induces the increase in glycolysis levels in the lungs of BPD mice. a : Detect the lactate levels in the BALF of N7, H7, N14 and H14 groups. b : The mRNA expression level of PKM2 quantified by RT-qPCR. c : The activity of HK in the lung tissue of N7, H7, N14 and H14 groups. d : The protein levels of PKM2 in the lung tissue of N7, H7, N14 and H14 groups were detected by WB. e : Representative images of immunofluorescence after staining with anti-CD86 antibody (green) and anti-PKM2 antibody (red) at 14 days. Nuclei were stained by DAPI (blue). f : The relative fluorescence intensity of PKM2 in the lungs of each group at 14 days. Data are shown as means ± SD ( n = 3). * P < 0.05, ** P <0.01, *** P<0.001

    Techniques Used: Expressing, Quantitative RT-PCR, Activity Assay, Immunofluorescence, Staining, Fluorescence



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    Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence <t>after</t> <t>staining</t> with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by <t>DAPI</t> (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001
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    Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence <t>after</t> <t>staining</t> with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by <t>DAPI</t> (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001
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    Image Search Results


    Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence after staining with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by DAPI (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001

    Journal: Inflammation

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia

    doi: 10.1007/s10753-026-02476-9

    Figure Lengend Snippet: Hyperoxia induces an increase in M1 type alveolar macrophages in BPD mice. a : WB was used to detect the levels of iNOS in the lung tissues of N7, H7, N14 and H14 groups, and quantitative analysis was conducted. b : The representative gating strategy of flow cytometry was used to identify CD45 + CD11c + SiglecF + CD86 + cells in lung tissues. c : The mean fluorescence intensity and percentage of CD86 of alveolar macrophages in each group at 7 days and 14 days was analyzed. d : Representative images of immunofluorescence after staining with anti-F4/80 antibody (yellow) and anti-CD86 antibody (green) at 14 days. Nuclei were stained by DAPI (blue). e : The relative immunofluorescence intensity of CD86 in the lungs of N14 and H14. Data are shown as means ± SD (n = 3). * P < 0.05, ** P <0.01, *** P <0.001

    Article Snippet: After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan).

    Techniques: Flow Cytometry, Fluorescence, Immunofluorescence, Staining

    Hyperoxia induces the increase in glycolysis levels in the lungs of BPD mice. a : Detect the lactate levels in the BALF of N7, H7, N14 and H14 groups. b : The mRNA expression level of PKM2 quantified by RT-qPCR. c : The activity of HK in the lung tissue of N7, H7, N14 and H14 groups. d : The protein levels of PKM2 in the lung tissue of N7, H7, N14 and H14 groups were detected by WB. e : Representative images of immunofluorescence after staining with anti-CD86 antibody (green) and anti-PKM2 antibody (red) at 14 days. Nuclei were stained by DAPI (blue). f : The relative fluorescence intensity of PKM2 in the lungs of each group at 14 days. Data are shown as means ± SD ( n = 3). * P < 0.05, ** P <0.01, *** P<0.001

    Journal: Inflammation

    Article Title: PKM2 Promotes Glycolysis in Alveolar Macrophages and Induces Inflammation in Bronchopulmonary Dysplasia

    doi: 10.1007/s10753-026-02476-9

    Figure Lengend Snippet: Hyperoxia induces the increase in glycolysis levels in the lungs of BPD mice. a : Detect the lactate levels in the BALF of N7, H7, N14 and H14 groups. b : The mRNA expression level of PKM2 quantified by RT-qPCR. c : The activity of HK in the lung tissue of N7, H7, N14 and H14 groups. d : The protein levels of PKM2 in the lung tissue of N7, H7, N14 and H14 groups were detected by WB. e : Representative images of immunofluorescence after staining with anti-CD86 antibody (green) and anti-PKM2 antibody (red) at 14 days. Nuclei were stained by DAPI (blue). f : The relative fluorescence intensity of PKM2 in the lungs of each group at 14 days. Data are shown as means ± SD ( n = 3). * P < 0.05, ** P <0.01, *** P<0.001

    Article Snippet: After washing, DAPI staining reagent (G1012, Servicebio, China) was added, and the sections were observed under an upright fluorescence microscope (Nikon, Japan).

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Immunofluorescence, Staining, Fluorescence